一、细胞基本属性 | |||||
产品名称 | PSMC1 knockout HeLa cell line | ||||
产品货号 | IMKO-H010 | ||||
产品规格 | 1×106cells/T25或1mL冻存管 | ||||
储存及运输 | 干冰运输;储存在液氮中 | ||||
产品分类 | Hela细胞 | ||||
物种 | 人 | ||||
修饰基因 | PSMC1 | ||||
修饰类型 | 基因敲除 | ||||
转导方法 | CRISPR/Cas9 | ||||
克隆类型 | 纯化克隆 | ||||
细胞鉴定 | 通过遗传学方法确认PSMC1基因敲除 | ||||
细胞形态 | 上皮细胞样 | ||||
生长特性 | 贴壁生长 | ||||
培养体系 | 90%MEM+10% FBS+1%PS | ||||
传代比例 | 1:2至1:3 | ||||
传代周期 | 每周3次 | ||||
培养条件 | 气相:95%空气+5%二氧化碳;温度:37℃ | ||||
冻存条件 | 无血清冻存液,液氮储存 | ||||
补充内容 | PSMC1是一种编码蛋白质的基因,与一些疾病如Birk-Aharoni综合征和Bjornstad综合征相关联。在人体中,PSMC1编码的蛋白质是19S蛋白酶体复合物的19个基本亚基之一,这个复合物在蛋白质降解中起着重要作用。 最近的研究表明,PSMC1变异可以导致一种新的神经系统综合征。通过分子遗传学研究发现,PSMC1的双等位基因变异会影响真核蛋白酶体基本单位的一个组成部分,进而引起这种疾病。对果蝇的功能性研究验证了这种变异的致病性,显示PSMC1 Drosophila同源基因的消除会完全逆转表型效应。 | ||||
构建方法 | |||||
PSMC1基因敲除细胞系的构建通常涉及的步骤 | 1.设计sgRNA(单指导RNA):选择目标PSMC1基因序列,并设计sgRNA用于精准敲除该基因。 2.选择适当的细胞系:选择适合的细胞系,通常选择易于转染和培养的细胞系。 3.转染sgRNA和Cas9:将设计好的sgRNA和Cas9基因导入目标细胞中,通常通过转染技术实现。 4.筛选敲除细胞系:利用细胞毒性筛选剂或选择性培养基对转染后的细胞进行筛选,筛选出成功敲除PSMC1基因的细胞系。 5.鉴定敲除细胞系:通过PCR、Western blot等技术验证PSMC1基因是否成功敲除,并确认细胞系中PSMC1蛋白的表达水平。 6.功能分析:对PSMC1敲除细胞系进行功能分析,比如观察其生长、增殖、代谢以及对特定刺激的反应等。 7. 维护和保存:维护敲除细胞系的培养,并进行合适的保存以备后续实验使用。 | ||||
PSMC1基因的基本信息 | Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
Human (Homo sapiens) | PSMC1 | 5700 | NM_002802.3 | NP_002793.2 | |
关于基因 | Official Symbol | PSMC1 | |||
Previous Symbol | PRS4 | ||||
Official Full Name | Proteasome 26S Subunit, ATPase 1 | ||||
Synonyms | P45, MOV-34, MGC13679 | ||||
Location | 14q32.11 | ||||
Gene Type | Protein Coding | ||||
Uniprot ID | B4DR63, P49014, P62191, Q03527, Q6IAW0, Q6NW36, Q96AZ3 | ||||
Pathway/Library | PSMC1 is involved in pathways related to protein degradation and cellular homeostasis. It plays a crucial role in the regulation of activated PAK-2p34 by proteasome-mediated degradation and the assembly of the pre-replicative complex. | ||||
Gene Summary | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit and a 20S core alpha subunit interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. This subunit also interacts with the adenovirus E1A protein and this interaction alters the activity of the proteasome. Finally, this subunit interacts with ataxin-7, suggesting a role for the proteasome in the development of spinocerebellar ataxia type 7, a progressive neurodegenerative disorder. [provided by RefSeq, Jul 2008] |
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